f nucleatum subsp polymorphum Search Results


f. nucleatum subsp. polymorphum jcm12990 t  (BioResource International Inc)
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Amplification of the targeted CRISPR region of F. nucleatum -type strains by F. nucleatum -strain genotyping PCR. ( a ) Electrophoresis pattern of PCR products of F. nucleatum -type strains. M, DNA ladder marker; an, F. nucleatum subsp. animalis ; nu, F. nucleatum subsp. nucleatum ; po, F. nucleatum subsp. <t>polymorphum</t> ; vi, F. nucleatum subsp. vincentii ; NC, negative control. The size of the marker fragments, from top to bottom, was 20 , 10, 7.0, 5.0 , 4.0, 3.0, 2.0 , 1.5, 1.3, 1.0 , 0.7, 0.5 , 0.4, 0.3, 0.2, and 0.1 kbp; the intensified fragments are underlined. ( b ) DNA sequences of the 5′- and 3′-ends of PCR amplicon of F. nucleatum subsp. animalis Type I-B1 [yellow square in ( a )] are shown and were used for the analysis. Repeat sequences are detected by CRISPRCasFinder and written in red. DNA sequences of other PCR amplicons are in .
F. Nucleatum Subsp. Polymorphum Jcm12990 T, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amplification of the targeted CRISPR region of F. nucleatum -type strains by F. nucleatum -strain genotyping PCR. ( a ) Electrophoresis pattern of PCR products of F. nucleatum -type strains. M, DNA ladder marker; an, F. nucleatum subsp. animalis ; nu, F. nucleatum subsp. nucleatum ; po, F. nucleatum subsp. polymorphum ; vi, F. nucleatum subsp. vincentii ; NC, negative control. The size of the marker fragments, from top to bottom, was 20 , 10, 7.0, 5.0 , 4.0, 3.0, 2.0 , 1.5, 1.3, 1.0 , 0.7, 0.5 , 0.4, 0.3, 0.2, and 0.1 kbp; the intensified fragments are underlined. ( b ) DNA sequences of the 5′- and 3′-ends of PCR amplicon of F. nucleatum subsp. animalis Type I-B1 [yellow square in ( a )] are shown and were used for the analysis. Repeat sequences are detected by CRISPRCasFinder and written in red. DNA sequences of other PCR amplicons are in .

Journal: Microbiology Spectrum

Article Title: Strain-level detection of Fusobacterium nucleatum in colorectal cancer specimens by targeting the CRISPR–Cas region

doi: 10.1128/spectrum.05123-22

Figure Lengend Snippet: Amplification of the targeted CRISPR region of F. nucleatum -type strains by F. nucleatum -strain genotyping PCR. ( a ) Electrophoresis pattern of PCR products of F. nucleatum -type strains. M, DNA ladder marker; an, F. nucleatum subsp. animalis ; nu, F. nucleatum subsp. nucleatum ; po, F. nucleatum subsp. polymorphum ; vi, F. nucleatum subsp. vincentii ; NC, negative control. The size of the marker fragments, from top to bottom, was 20 , 10, 7.0, 5.0 , 4.0, 3.0, 2.0 , 1.5, 1.3, 1.0 , 0.7, 0.5 , 0.4, 0.3, 0.2, and 0.1 kbp; the intensified fragments are underlined. ( b ) DNA sequences of the 5′- and 3′-ends of PCR amplicon of F. nucleatum subsp. animalis Type I-B1 [yellow square in ( a )] are shown and were used for the analysis. Repeat sequences are detected by CRISPRCasFinder and written in red. DNA sequences of other PCR amplicons are in .

Article Snippet: Type strains, F. nucleatum subsp. animalis JCM11025 T , F. nucleatum subsp. nucleatum JCM8532 T , F. nucleatum subsp. polymorphum JCM12990 T , and F. nucleatum subsp. vincentii JCM11023 T , were purchased from the Japan Collection of Microorganisms of RIKEN BioResource Research Center.

Techniques: Amplification, CRISPR, Electrophoresis, Marker, Negative Control

Detection of F. nucleatum strains from saliva of CRC patients by the F. nucleatum -strain genotyping PCR. ( a ) Detection of PCR amplicons from single and mixed multiple genomic DNA of F. nucleatum strains. From left to right, F. nucleatum subsp. animalis A3, A4, A5, A7 (Type I-B1); F. nucleatum subsp. polymorphum A-S11, P3, P7 (Type I-B2); F. nucleatum subsp. animalis A1, A8, F. nucleatum subsp. vincentii V7, F. nucleatum subsp. nucleatum N1 (Type II-A); F. nucleatum subsp. animalis A1, A3, F. nucleatum subsp. polymorphum P4 (Type III-A). Origins of many isolates are shown in Fig. S2. Strains A7, A8, and V7, A-S11, and A1, which are not in Fig. S2, were isolated from saliva of Patient H, saliva of Patient A, and CRC of Patient C, respectively, in our previous study . ( b ) Single genomic DNA of isolate or total genomic DNA extracted from saliva (the origin of the isolates) was used as a template (Patient M). The lane of total saliva (left, “S” in yellow) contains the bands identified in the isolates (blue arrowhead) and the amplicons derived from other non-isolates (pink arrowhead). Data are not available for strain V2 of Patient E and A12 of Patient M because these do not have all CRISPR types (see ).

Journal: Microbiology Spectrum

Article Title: Strain-level detection of Fusobacterium nucleatum in colorectal cancer specimens by targeting the CRISPR–Cas region

doi: 10.1128/spectrum.05123-22

Figure Lengend Snippet: Detection of F. nucleatum strains from saliva of CRC patients by the F. nucleatum -strain genotyping PCR. ( a ) Detection of PCR amplicons from single and mixed multiple genomic DNA of F. nucleatum strains. From left to right, F. nucleatum subsp. animalis A3, A4, A5, A7 (Type I-B1); F. nucleatum subsp. polymorphum A-S11, P3, P7 (Type I-B2); F. nucleatum subsp. animalis A1, A8, F. nucleatum subsp. vincentii V7, F. nucleatum subsp. nucleatum N1 (Type II-A); F. nucleatum subsp. animalis A1, A3, F. nucleatum subsp. polymorphum P4 (Type III-A). Origins of many isolates are shown in Fig. S2. Strains A7, A8, and V7, A-S11, and A1, which are not in Fig. S2, were isolated from saliva of Patient H, saliva of Patient A, and CRC of Patient C, respectively, in our previous study . ( b ) Single genomic DNA of isolate or total genomic DNA extracted from saliva (the origin of the isolates) was used as a template (Patient M). The lane of total saliva (left, “S” in yellow) contains the bands identified in the isolates (blue arrowhead) and the amplicons derived from other non-isolates (pink arrowhead). Data are not available for strain V2 of Patient E and A12 of Patient M because these do not have all CRISPR types (see ).

Article Snippet: Type strains, F. nucleatum subsp. animalis JCM11025 T , F. nucleatum subsp. nucleatum JCM8532 T , F. nucleatum subsp. polymorphum JCM12990 T , and F. nucleatum subsp. vincentii JCM11023 T , were purchased from the Japan Collection of Microorganisms of RIKEN BioResource Research Center.

Techniques: Isolation, Derivative Assay, CRISPR